The book is a manual of protocols for working with protozoa with chapters written by experts in the study of various protozoa (both free living and parasitic). It is one of the few sources for media and isolation techniques for these organisms. The format allows removal of pages for photocopy for use as teaching or bench laboratory aids. Major sections include chapters related to isolation, cultivation, and cryopreservation of protozoa; ecological methods; fixation, staining, light and electron microscopic techniques for protozoa; molecular analysis of protozoa; and educational experiments and demonstrations using protozoa.