This book collects the most effective and cutting-edge methodsand protocols for deriving and culturing human embryonic and adultstem cells?in one handy resource. This groundbreaking book follows the tradition of previousbooks in the Culture of Specialized Cells Series?eachmethods and protocols chapter is laid out exactly like the next,with stepwise protocols, preceded by specific requirements for thatprotocol, and a concise discussion of methods illustrated by data.The editors describe a limited number of representative techniquesacross a wide spectrum of stem cells from embryonic, newborn, andadult tissue, yielding an all-encompassing and versatile guide tothe field of stem cell biology and culture. The book includes a comprehensive list of suppliers for allequipment used in the protocols presented, with websites availablein an appendix. Additionally, there is a chapter on qualitycontrol, and other chapters covering legal and ethical issues,cryopreservation, and feeder layer culture. This text is aone-stop resource for all researchers, clinical scientists,teachers, and students involved in this crucial area of study.